The increased number of clinics and hospitals in Bangladesh is resulting in the increased amount of waste generation. Hospital waste contains toxic chemicals and hazardous materials from several diagnostic and treatment processes. The improper disposal of hospital waste in the country poses a high health risk to humans as well as the environment. The improvement of waste management in clinics and hospitals is essential to minimize the spread of infectious diseases. The Association for Rural Development Studies (ARDS), in collaboration with BRAC, has undertaken this study to formulate guidelines and recommending a safe handling system of hospital waste in Bangladesh. A survey was conducted on the characteristics of hospital waste and the present practice of hospital waste management in 38 hospitals and clinics in Dhaka, Mymensingh and Dinajpur. The results revealed that the present waste disposal system was unhygienic and unsafe. The knowledge level of hospital staff on the harmful impacts of improper waste disposal was also very low. The need for raising awareness, improving waste handling and disposal system, and implementing laws and regulations regarding hospital waste management are recommended.